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Comparative bioinformatic research of gene promoters of DREB2B transcription factors in Deschampsia antarctica and some other cereals

Bublyk O.M., Andreev I.O., Kunakh V.A.


SUMMARY. The organization of promoters of DREB2B TF orthologous genes involved in the response to abiotic stress was studied in extremophile plant D. antarctica E. Desv and 12 other grass species with different cold and drought resistance. The average evolutionary distances were 0.621 between the promoter sequences and 0.442 between coding sequences, including introns; the values of nucleotide diversity (π) for these regions were 0.410 and 0.274, respectively. Clustering of sequences was generally consistent with the accepted taxonomy of the Poaceae family. In total, 54 cis-elements involved in the response to abiotic and biotic stresses, light, hormones, such as abscisic acid, auxin, methyl jasmonate, ethylene, gibberellin and salicylic acid, and tissue-specific cis elements were identified. A large proportion of these cis elements were associated with abiotic stress response that is consistent with known functions of DREB2B TF. Except for a few isolated differences, the studied grass species of different subfamilies and D. antarctica had a similar set of cis elements in the DREB2B promoter, a finding that indicates the similarity in the control of this gene expression and its potential functions in these species.

Key words: abiotic and biotic stress, DREB2B transcription factors, cis regulatory elements, grasses

Tsitologiya i Genetika 2022, vol. 56, no. 5, pp. 3-15

  • Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv, 03143 Ukraine

E-mail: o.m.bublyk

Bublyk O.M., Andreev I.O., Kunakh V.A. Comparative bioinformatic research of gene promoters of DREB2B transcription factors in Deschampsia antarctica and some other cereals, Tsitol Genet., 2022, vol. 56, no. 5, pp. 3-15.

In "Cytology and Genetics":
O. M. Bublyk, I. O. Andreev & V. A. Kunakh Comparative Analysis of Promoters of DREB2B Transcription Factor Genes in Deschampsia antarctica and Other Grasses, Cytol Genet., 2022, vol. 56, no. 5, pp. 399–409
DOI: 10.3103/S0095452722050048


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