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Comparison of alleles at Gli-2 loci of common wheat by means of two-dimensional electrophoresis of gliadin
SUMMARY. Electrophoretic mobility (EM) and molecular weight (MW) of some allelic variants of α- and β-gliadins contrlled by Gli-2 loci were compared by means of two-dimensional (APAGE × SDS) electrophoresis. Com-parison of α-gliadins of the alleles Gli-A2b and Gli-A2p, of β-gliadins of the Gli-B2b and Gli-B2c, and of β-gliadins of the Gli-D2b, Gli-D2c, Gli-D2j, and Gli-D2r indicated that a gliadin with lower EM had, has a rule, bigger MW which is known to depend on the length of the polyglutamine domain of gliadin of α-type. However, allelic variants of the α-gliadin encoded by Gli-D2b è Gli-D2e differ in EM but not in apparent MW. It might be caused by a substitution of some charged/uncharged aminoacids in the polypeptide of gliadin. Allele Gli-B2o which is very frequent in up-to-date common wheat germplasm originated probably by means of unequal crossingover. Some alleles at Gli-A2 is found to control completely different blocks of gliadins and therefore might come to common wheat from different genotypes of the polymorphic diploid donor of the A genome. The results indicate that the reason of the known more vast polymorphism of gliadins controlled by Gli-2 loci as compared with Gli-1 loci is the considerable difference of the structure, first, of Gli-1 and Gli-2 loci (Gli-2 loci have more expressed genes per locus) and, second, of genes encoding gliadins of α- and γ-types (α-gliadins are shown to contain a long polyglutamine sequences highly variable in their length).
Key words: common wheat, gliadin, alleles at Gli-2 loci, two-dimensional electrophorsis
Tsitologiya i Genetika 2018, vol. 52, no. 2, pp. 3-12
E-mail: emetakovsky gmail.com
Metakovsky E.V., Melnik V.A., Redaelli R., Rodriguez-Quijano M. Comparison of alleles at Gli-2 loci of common wheat by means of two-dimensional electrophoresis of gliadin, Tsitol Genet., 2018, vol. 52, no. 2, pp. 3-12.
In "Cytology and Genetics":
E. V. Metakovsky, V. A. Melnik, R. Redaelli, M. Rodriguez-Quijano Comparison of Alleles at Gli-2 Loci of Common Wheat by Means of Two-Dimensional Electrophoresis of Gliadin, Cytol Genet., 2018, vol. 52, no. 2, pp. 87–94
Metakovsky, E.V. and Graybosch, R.A., Gliadin alleles in wheat: identification and application, in Gliadin and Glutenin. The Unique Balance of Wheat Quality
, Wrigley, C., Bekes, F., and Bushuk, W., Eds., AACC Internat., 2006, pp. 85–114.CrossRefGoogle Scholar
Sozinov, A.A. and Poperelya, F.A., Genetic classification of prolamins and its use for plant breeding, Ann. Technol. Agric.
, 1980, vol. 29, pp. 229–245.Google Scholar
Sobko, T.A. and Poperelya, F.A., Frequencies of occurrence of alleles of gliadin-coding loci in winter common wheat, Visn. S.-G. Nauki
, 1986, no. 5, pp. 84–87.Google Scholar
Metakovsky, E.V., Gliadin allele identification in common wheat. 2. Catalogue of gliadin alleles in common wheat, J. Genet. Breed.
, 1991, vol. 45, no. 4, pp. 325–344.Google Scholar
Metakovsky, E.V., Davydov, S.D., Chernakov, V.M., and Upelniek, V.P., Gliadin allele identification in common wheat. 3. Frequency of occurrence and appearance of spontaneous mutations at the gliadin-coding loci, J. Genet. Breed.
, 1993, vol. 47, no. 3, pp. 221–236.Google Scholar
Upelniek, V.P., Novoselskaya, A.Yu., Sutka, J., Galiba, G., and Metakovsky, E.V., Genetic variation at storage protein coding loci of common wheat (cv Chinese Spring) induced by nitrosoethylurea and by cultivation of immature embryos in vitro, Theor. Appl. Genet.
, 1995, vol. 90, nos. 3–4, pp. 372–379.PubMedGoogle Scholar
Kasarda, D.D., Okita, T.W., Bernardin, J.E., Baecker, P.A., Nimmo, C.C., Lew, J.L., Dietler, M.D., and Green, F.C., Nucleic acid (cDNA) and amino acid sequence of α-type gliadins from wheat (Triticum aestivum
), Proc. Nat. Acad. Sci. U. S. A.
, 1984, vol. 81, no. 15, pp. 4712–4716.CrossRefGoogle Scholar
Barak, S., Mudgil, D., and Khatkar, B.S., Biochemical and functional properties of wheat gliadins: a review, Crit. Rev. Food Sci. Nutr.
, 2015, vol. 55, no. 3, pp. 357–368.CrossRefPubMedGoogle Scholar
Metakovsky, E.V., Wheat Grain Storage Proteins: Classical Genetics, Mutations, Phylogeny, Seed Breeding, and Grain Quality, Lambert Acad. Publ.
, 2015, 320 p.Google Scholar
Anderson, O.D. and Greene, F.C., The α-gliadin gene family. 2. DNA and protein sequence variation, subfamily structure, and origins of pseudogenes, Theor. Appl. Genet.
, 1997, vol. 95, no. 1, pp. 59–65.CrossRefGoogle Scholar
Li, J., Wang, S.-L., Cao, D.-W., Subburaj, L.S., Li, X.H., Zeller, F.J., Hsam, S.L.K., and Yan, Y.-M., Cloning, expression, and evolutionary analysis of α-gliadin genes from Triticum
genomes, J. Appl. Genet.
, 2013, vol. 54, no. 2, pp. 157–167.CrossRefPubMedGoogle Scholar
Li, Y., Xin, R., Zhang, D., and Li, S., Molecular characterization of α-gliadin genes from common wheat cultivar Zhengmai 004 and their role in quality and celiac disease, Crop J.
, 2014, vol. 2, no. 1, pp. 10–21.CrossRefGoogle Scholar
Noma, S., Kawaura, K., Hayakawa, K., Abe, C., Tsuge, N., and Ogihara, J., Comprehensive molecular characterization of the α/β-gliadin multigene family in hexaploid wheat, Mol. Genet. Genom.
, 2016, vol. 291, no. 1, pp. 65–77.CrossRefGoogle Scholar
Metakovsky, E.V., Melnik, V.A., Vaccino, P., and Rodriguez-Quijano, M., Comparison of alleles at Gli-1 loci of common wheat by means of two-dimensional electrophoresis of gliadin and RFLP analysis, Cytol. Genet.
, 2018, vol. 52, no. 1, pp. 16–27.CrossRefGoogle Scholar
Sapirstein, H.D. and Bushuk, W., Computer-aided wheat cultivar identification and analysis of densitometric scanning profiles of gliadin electrophoregrams, Seed Sci. Technol.
, 1986, vol. 14, no. 3, pp. 489–517.Google Scholar
Koval, S.F. and Metakovsky, E.V., Adaptive value of some quantitative and qualitative characters in the experimental hybrid population of T. aestivum, Selskokhoz. Biol.
, 1985, no. 11, pp. 48–52.Google Scholar
Huang, Z., Long, H., Wei, Y.-M., Yan, Z.H., and Zheng, Y.L., Allelic variations of α-gliadin genes from species of Aegilops
and insights into evolution of α-gliadin multigene family among Triticum
, 2016, vol. 144, no. 2, pp. 213–222.Google Scholar
Metakovsky, E.V. and Sozinov, A.A., Genetics of gliadin proteins and the problems of interpreting results obtained with somaclonal variation in wheat, in Biotechnology in Agriculture and Forestry (Wheat), Bajaj, Y.P.S., Ed.
, 1990, vol. 13, pp. 526–537.Google Scholar
Vaccino, P. and Metakovsky, E.V., Gliadin alleles in DNA RFLP patterns of common wheat: implication for analysis of organization and evolution of complex loci, Theor. Appl. Genet.
, 1995, vol. 90, no. 2, pp. 173–181.CrossRefPubMedGoogle Scholar
Gu, Y.Q., Crossman, C., Kong, X., Luo, M., You, F.M., Coleman-Derr, D., and Dubcovsky, J., Genomic organization of the complex α-gliadin gene loci in wheat, Theor. Appl. Genet.
, 2004, vol. 109, no. 3, pp. 648–657.CrossRefPubMedGoogle Scholar
Zmienko, A., Samelak, A., Kozlowski, P., and Figlerowicz, M., Copy number polymorphism in plant genomes, Theor. Appl. Genet.
, 2015, vol. 127, no. 1, pp. 1–18.CrossRefGoogle Scholar
Qi, P.F., Wei, Y.M., Ouellet, T., Chen, Q., Tan, X., and Zheng, Y.L., The γ-gliadin multigene family in common wheat (Triticum aestivum
) and its closely related species, BMC Genom.
, 2009, vol. 10, no. 1, pp. 168–181.CrossRefGoogle Scholar
Wang, S., Shen, X., Ge, P., Saminathan, J.L., Li, S.X., Zeller, F.J., Hsam, K.L., and Yan, Y., Molecular characterization and dynamic expression patterns of two types of γ-gliadin genes from Aegilops
species, Theor. Appl. Genet.
, 2012, vol. 125, no. 7, pp. 1371–1384.CrossRefPubMedGoogle Scholar
Raquin, A.-L., Depaulis, F., Lambert, A., Galic, N., Brabant, P., and Goldringer, I., Experimental estimation of mutation rates in a wheat population with a gene genealogy approach, Genetics
, 2008, vol. 179, no. 4, pp. 2195–2211.CrossRefPubMedPubMedCentralGoogle Scholar