TSitologiya i Genetika 2014, vol. 48, no. 1, 3-9
Cytology and Genetics 2014, vol. 48, no. 1, 1-6, doi: https://www.doi.org/10.3103/S0095452714010095

Structural organization of 5S ribosomal DNA of Rosa rugosa

Tynkevich Y.O., Volkov R.A.

SUMMARY. In order to clarify molecular organization of the genomic region encoding 5S rRNA in diploid species Rosa rugosa several 5S rDNA repeated units were cloned and sequenced. Analysis of the obtained sequences revealed that only one length variant of 5S rDNA repeated units, which contains intact promoter elements in the intergenic spacer region (IGS) and appears to be transcriptionally active is present in the genome. Additionally, a limited number of 5S rDNA pseudogenes lacking a portion of coding sequence and the complete IGS was detected. A high level of sequence similarity (from 93,7 to 97,5 %) between the IGS of major 5S rDNA variants of East Asian R. rugosa and North American R. nitida was found indicating comparatively recent divergence of these species.

Keywords: 5S rDNA, molecular evolution ant taxonomy, Rosa

TSitologiya i Genetika
2014, vol. 48, no. 1, 3-9

Current Issue
Cytology and Genetics
2014, vol. 48, no. 1, 1-6,
doi: 10.3103/S0095452714010095

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