Obtaining the transgeniс lines of finger millet Eleusine coracanа (L.) Gaertn. with dinitroaniline resistance

Bayer G.Ya., Yemets A.I., Blume Ya.B.

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SUMMARY. The current data is dedicated to the study of bioballistic and Agrobacterium-mediated transformation of finger millet with the constructs carrying the mutant α-tubulin gene (Tuam1), isolated from R-biotype goosegrass (Eleusine indica L.), for the decision of problem of dinitroaniline-resistance. It was found that 10 µМ of trifluralin is optimal for the selection of transgene plants of finger millet. PCR analysis of transformed lines confirmed the transgene nature of plants. The analysis of seed of Т₁ of transgene lines confirmed heterozygous character of inheritance of the resistance.

Key words: finger millet, genetic transformation, mutant α-tubulin, resistance, dinitroanilines

Tsitologiya i Genetika 2014, vol. 48, no. 3, pp. 3-11

E-mail: galinabayer mail.ru

Bayer G.Ya., Yemets A.I., Blume Ya.B. Obtaining the transgeniс lines of finger millet Eleusine coracanа (L.) Gaertn. with dinitroaniline resistance, Tsitol Genet., 2014, vol. 48, no. 3, pp. 3-11.

In "Cytology and Genetics":
G. Ya. Bayer, A. I. YemetsYa. B. Blume Obtaining the transgenic lines of finger millet Eleusine coracana (L.). with dinitroaniline resistance, Cytol Genet., 2014, vol. 48, no. 3, pp. 139–144
DOI: 10.3103/S0095452714030025