Genetic transformation of the moss Ceratodon purpureus by novel polyсationic carriers of DNA

Finiuk N.S., Chaplya A.Y., Mitina N.Y., Boiko N.M., Lobachevska O.V., Miahkota О.S., Yemets A.I., Blume Ya.B., Zaichenko O.S., Stoika R.S.

There is a big progress in application of genetic engineering for improving the biological properties of different organisms. Viral and non-viral carriers are used for delivery of genetic material into target cells. Nanoscale polymeric materials of natural and synthetic origin are the most promising gene delivery agents. These polymers have demonstrated high efficiency of DNA delivery into the mammalian cells, although they were not very effective in plant cells. Here, the procedure for genetic transformation of Ceratodon purpureus (Hedw.) Brid. moss protoplasts is described. Method is based on the application of novel surface-active polymeric carriers of the polyDMAEM structure and controlled length and charge. It allows obtaining more transient and stable moss transformants per microgram of plasmid DNA when compared with known protocol based on using polyethyleneglycol. It is easier, more convenient, and cheaper than the «gene gun» method. Perspectives for further improvement of structure and functional characteristics of novel polymeric carriers are considered for delivery of genetic material into plant cells.

Key words: genetic transformation of protoplasts, polycationic carriers of DNA, moss Ceratodon purpureus

Tsitologiya i Genetika 2014, vol. 48, no. 6, pp. 3-10

E-mail: stoika cellbiol.lviv.ua

Finiuk N.S., Chaplya A.Y., Mitina N.Y., Boiko N.M., Lobachevska O.V., Miahkota О.S., Yemets A.I., Blume Ya.B., Zaichenko O.S., Stoika R.S. Genetic transformation of the moss Ceratodon purpureus by novel polyсationic carriers of DNA, Tsitol Genet., 2014, vol. 48, no. 6, pp. 3-10.