Creation of cDNA library from meristem of finger millet panicle

Radchuk V., Pirko Ya.V., Isayenkov S.V., Yemets A.I., Blume Ya.B.

SUMMARY. The protocol for production of full-size cDNA using SuperScript® Full-Length cDNA Library Construction Kit II (Invitrogen) was tested and high quality cDNA library from meristematic tissue of finger millet panicle (Eleusine coracana (L.) Gaertn) was created. The titer of obtained cDNA library comprised 3,01·10⁵ CFU/ml in avarage. In average the length of cDNA insertion consisted about 1070 base pairs, the effectivity of cDNA fragment insertions – 99,5 %. The selective sequencing of cDNA clones from created library was performed. The sequences of cDNA clones were identified with usage of BLAST- search. The results of cDNA library analysis and selective sequencing represents prove good functionality and full length character of inserted cDNA clones. Obtained cDNA library from meristematic tissue of finger millet panicle represents good and valuable source for isolation and identification of key genes regulating metabolism and meristematic development and for mining of new molecular markers to conduct out high quality genetic investigations and molecular breeding as well.

Tsitologiya i Genetika 2014, vol. 48, no. 5, pp. 3-10

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Radchuk V., Pirko Ya.V., Isayenkov S.V., Yemets A.I., Blume Ya.B. Creation of cDNA library from meristem of finger millet panicle, Tsitol Genet., 2014, vol. 48, no. 5, pp. 3-10.