EFFECT OF INHIBITORS SERINE/THREONINE PROTEIN KINASES AND PROTEIN PHOSPHATASES ON MITOSIS PROGRESSION OF SYNCHRONIZED TOBACCO BY-2 CELLS

Sheremet Ya.A., Yemets A.I., Azmi A., Vissenberg K., Verbelen J.-P., Blume Ya.B.

SUMMARY. In order to investigate the role of various serine/threonine protein kinases and protein phosphatases in the regulation of mitosis progression in plant cells the influence of cyclin-dependent (olomoucine) and Ca²⁺-calmodulin-dependent (W7) protein kinases inhibitors, as well as protein kinase C inhibitors (H7 and staurosporine) and protein phosphatases inhibitor (okadaic acid) on mitosis progression in synchronized tobacco BY-2 cells has been studied. It was found that BY-2 culture treatment with inhibitors of cyclin dependent protein kinases and protein kinase C causes prophase delay, reduces the mitotic index and displaces of mitotic peak as compare with control cells. Inhibition of Ca²⁺-calmodulin dependent protein kinases enhances the cell entry into prophase and delays their exit from mitosis. Meanwhile inhibition of serine/threonine protein phosphatases insignificantly enhances of synchronized BY-2 cells entering into all phases of mitosis.

Tsitologiya i Genetika 2012, vol. 46, no. 2, pp. 3-11

• Институт пищевой биотехнологии и геномики НАН Украины, Киев
• Университет Антверпена, отдел биологии роста и развития растений, Бельгия

E-mail: yarasheremet gmail.com

Sheremet Ya.A., Yemets A.I., Azmi A., Vissenberg K., Verbelen J.-P., Blume Ya.B. EFFECT OF INHIBITORS SERINE/THREONINE PROTEIN KINASES AND PROTEIN PHOSPHATASES ON MITOSIS PROGRESSION OF SYNCHRONIZED TOBACCO BY-2 CELLS, Tsitol Genet., 2012, vol. 46, no. 2, pp. 3-11.