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Hete- rogeneity of cell population of lymphoma NK/Ly and leukemia L-1210 according to carbohydrate structure of cell surface : immunocytochemical analysis of lectin binding
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The heterogeneity of tumor cell populations according to binding of lectins from lentil (LcL), wheat germs (WGA), peanut (PNA) and concanavalin A was investigated on a model of murine Nemeth-Kellner lymphoma (NK/Ly) and leukemia L-1210. Bound lectins were detected by indirect immunochemical method using home obtained polyclonal antilectin antibodies and immunogold silver staining (IGSS) technique. Significant differences in binding of Con A were revealed between NK/Ly (67 % Con A+) and L-1210 (7,2 % Con A+) cells, while the differences in binding of other lectins with both types of tumor cells were not significant. A relatively high percentage of PNA+ cells was registered that can indicate a high degree of desialization of membrane glycoproteins.
Key words: lymphoma NK/Ly, leukemia L 1210, carbohydrates of cell surface, lectins
Tsitologiya i Genetika 2011, vol. 45, no. 2, pp. 3-9
E-mail: lootsik cellbiol.lviv.ua, stoika cellbio
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