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Development of specific and degenerated primers to CesA genes encoding flax (Linum usitatissimum L.) cellulose syntase
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Cellulose synthase catalytic subunit genes, CesA, have been discovered in several higher plant species, and it has been shown that the CesA gene family has multiple members. HVR2 fragment of these genes determine the class specificity of the CESA protein and it´s participation in the primary or secondary cell wall synthesis. The aim of this study was development of specific and degenerated primers to flax CesA gene fragments leading to obtaining the class specific HVR2 region of the gene. Two pairs of specific primers to the certain fragments of ÑesA-1 and ÑesA-6 genes and one pair of degenerated primers to HVR2 region of all flax ÑesA genes were developed basing on comparison of six CesA EST sequences of flax and full cDNA sequences of Arabidopsis, poplar, maize and cotton plants, obtained from GenBank. After amplification of flax cDNA, the bands of expected size were detected (201 and 300 b.p. for the ÑesA-1 and ÑesA-6, and 600 b.p. for the HVR2 region of ÑesA respectively). The developed markers can be used for cloning and sequencing of flax ÑesA genes, identifying their number in flax genome, tissue and stage specificity.
Key words: cellulose syntase, CesA, degenerated primers, sequencing
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|Coded & Designed by Volodymyr Duplij||Modified 20.09.21|