Pleiotropic effects of chitinase gene from Serratia plymuthica> in transgenic potato

Shakhbazau A.V., Yakovleva G.A., Rodkina I.A., Kartel N.A.

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We estimate the influence of transgenic bacterial chitinase from soilborne Serratia plymuthica onto agronomical important traits of potato, such as productivity and non-specific resistance. Transgene has been delivered into potato variety Delfin via Agrobacterial transformation with pGreen0229 and pBI121-based vectors. Growth inhibition of chitin-containing pathogenic fungi was shown. However, over 40 % of transgenic lines demonstrated decreased non-specific resistance to late blight (down to 62 % compared to control genotype), as well as 40–60 % productivity drop.

Key words: chitinase, transgene, potato, transformation, pleiotropic effects

Tsitologiya i Genetika 2008, vol. 42, no. 2, pp. 3-9

E-mail: a.shakhbazau igc.bas-net.by, y_galina ma

Shakhbazau A.V., Yakovleva G.A., Rodkina I.A., Kartel N.A. Pleiotropic effects of chitinase gene from Serratia plymuthica> in transgenic potato, Tsitol Genet., 2008, vol. 42, no. 2, pp. 3-9.