Nicotiana sylvestris L. mutants resistant to isopropyl-N-phenylcarbamate possess an increased stability of the centers of microtubule organization

Stemlakh O.A., Yemets A.I., Blume Ya.B.

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By in vitro selection using γ-irradiation the N. sylvestris mutants resistant to isopropyl-N-phenylcarbamate (IPC) – herbicide from phenylcarbamate class have been obtained. Maximal concentration, at which N. sylvestris mutant lines were able to regenerate and induce root formation under selective pressure, was concentration of 30 mkМ IPC. IPC- resistance of mutants was confirmed by different tests, in particular by tests on an ability of their leaf explants to regenerate plantlets, and of their callus cells to survive on media with selective concentration of IPC; and also by genetic, morphometric, cytological and immunofluorescence analyses. The obtained results shown an increased resistance of mutant lines to this antimitotic drug action as compared to the control. It was shown that an increased resistance of MTOCs in the cells of these lines is a basics of IPC- resistance formation. It was established that aсquired trait of the resistance is inherited in F₁ and F₂ generations of mutants as dominant nuclear trait.

Tsitologiya i Genetika 2007, vol. 41, no. 6, pp. 3-10

E-mail: stelmakh univ.kiev.ua

Stemlakh O.A., Yemets A.I., Blume Ya.B. Nicotiana sylvestris L. mutants resistant to isopropyl-N-phenylcarbamate possess an increased stability of the centers of microtubule organization, Tsitol Genet., 2007, vol. 41, no. 6, pp. 3-10.