Obtaining of intertribal Brassica juncea+Arabidopsis thaliana somatic hybrids and study of transgenic trait behaviour

Ovcharenko O.A., Komarnitsky I.K., Cherep N.N., Gleba Yu.Yu., Kuchuk N.V.

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Intertribal somatic hybridization between wild type Brassica juncea (L.) Czern. & Coss. and transgenic A. thaliana L. has been carried out. Genome of A. thaliana plants contained heterologous transposable element Spm/dSpm, reporter GUS gene, selective genes for kanamycin- (npt II) and phosphinothricin (bar) resistance. Hybrid nature of obtained plants was confirmed with their morphology, GUS hystochemical assay, PCR-RFLP, RAPD and isozyme analyses.

It was determined that heterologous transposable element Spm/dSpm is able to functionate in hybrid plants. There was no complete elimination of A. thaliana genetic material in the hybrids and the transgenes were stably maintained.

Tsitologiya i Genetika 2004, vol. 38, no. 3, pp. 3-8

Ovcharenko O.A., Komarnitsky I.K., Cherep N.N., Gleba Yu.Yu., Kuchuk N.V. Obtaining of intertribal Brassica juncea+Arabidopsis thaliana somatic hybrids and study of transgenic trait behaviour, Tsitol Genet., 2004, vol. 38, no. 3, pp. 3-8.