Цитологія і генетика 2019, том 53, № 3, 72-74
Cytology and Genetics 2019, том 53, № 3, 239–249, doi: https://www.doi.org/10.3103/S0095452719030101

Genetic modification of Saccharum officinarum for herbicide tolerance

Muhammad Fahad Khan, Qurban Ali, Muhammad Tariq, Shafique Ahmed, Zahida Qamar, Idrees Ahmad Nasir

  1. Centre of Excellence in Molecular Biology, University of the Punjab Lahore, Pakistan
  2. Institute of Molecular Biology and Biotechnology, University of Lahore, Lahore Pakistan

РЕЗЮМЕ. Цукрова тростина – це одна з найважливіших сільськогосподарських рослин у світі, яка належить до родини Poaeceae, роду Saccharum. Дане дослідження було проведене у Лабораторії біотехнології насіння Центру передових технологій у сфері молекулярної біології Університету Пенджабу, м. Лахор, Пакистан. Мета дослідження полягала у зміні геному лінії цукрової тростини CPF246 за допомогою гену резистентності до гербіцидів GTGene за участі промотора убіквітину цукрової тростини та підтвердження за допомогою ПЦР і дот-блот-аналізу, а також ІФА аналізу експресії білку. Результати продемонстрували успішну трансформацію та експресію трансгенів стійкості до гліфосату. Це може стати значною віхою для дослідження, зосередженого на покращенні деяких властивостей Saccharum officinarum. Результати цього дослідження будуть корисними для скорочення вартості витрат на технології боротьби з бур’янами. Успіх цього дослідження відкриває нові горизонти для подальшого вдосконалення інших властивостей цукрової тростини, використовуючи генетичну модифікацію подібно до поточного дослідження.

Ключові слова: Saccharum officinarum, генетична модифікація, гербіцид, GTGene, гліфосат

Цитологія і генетика
2019, том 53, № 3, 72-74

Current Issue
Cytology and Genetics
2019, том 53, № 3, 239–249,
doi: 10.3103/S0095452719030101

Повний текст та додаткові матеріали

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