TSitologiya i Genetika 2020, vol. 54, no. 5, 75-88
Cytology and Genetics 2020, vol. 54, no. 5, 437–448, doi: https://www.doi.org/10.3103/S0095452720050096

Evaluation of phytotoxicity and mutagenicity of novel DMAEMA-containing gene carriers

Finiuk N., Romanyuk N., Mitina N., Lobachevska O., Zaichenko A., Terek O., Stoika R.

  1. Institute of Cell Biology of National Academy of Sciences of Ukraine, Drahomanov st., 14/16, Lviv, 79005, Ukraine
  2. Ivan Franko National University of Lviv, Hrushevskyy st., 4, Lviv, 79005, Ukraine
  3. Lviv Polytechnic National University, Bandera st., 12, Lviv, 79013, Ukraine
  4. Institute of Ecology of the Carpathians of National Academy of Sciences of Ukraine, Kozelnytska st., 4, Lviv, 79026, Ukraine

SUMMARY. A use of novel carriers for gene delivery is rapidly gro-wing, thus, investigation of potential phytotoxic and mutagenic action of gene delivery carriers is important for excluding their negative side effects. We found that poly-DMAEMA carriers used in 0,0025 % dose exhibited weak cytotoxic effect towards Allium cepa plant. In higher dose (0,025 %), they slightly (by 26–55 %) increased the level of catalase activity, but they did not affect the level of superoxide dismutase activity and malonic dialdehyde content in roots of A. cepa. The results of ana-telophase test in A. cepa did not demonstrate a genotoxic activity of the polymeric carriers used in 0.0025 % concentration and its higher dose (0,025 %). Slight genotoxic activity was detected only for BGP24 and BGP26, PEG-containing poly-DMAEMA carriers used in 0,025 %. The DMAEMA-based polymers did not possess muta-genic potential estimated in Ames test (–S9 and +S9). Thus, low phytotoxicity and absence of mutagenic action of novel polymeric carriers suggest their potential as promising nanocarriers for gene delivery into plant cells.

Keywords: poly(2-dimethylamino)ethyl methacrylate, polymeric carrier, ana-telophase assay, Ames test, catalase, superoxide dismutase, malonic dialdehyde

TSitologiya i Genetika
2020, vol. 54, no. 5, 75-88

Current Issue
Cytology and Genetics
2020, vol. 54, no. 5, 437–448,
doi: 10.3103/S0095452720050096

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