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Identification of Oryza sativa awn development regulatory genes orthologs in Triticinae accessions

Navalikhina A., Antonyuk M., Pasichnyk T., Ternovska T.

 




SUMMARY. Information on the genetic control of the awn development in the bread wheat is currently limited to the identification of three genes Ц Hd, B1, and B2 awnedness supressors, and no promotors have yet been identified. Another Gramineae species, Oryza sativa, has more that ten genes involved in the awn morphogenesis. This article presents the results of the wheat genome sequence analysis for the search of genes, orthologous to the known awn development regulators in rice Ц TOB1, ETT2 and DL. With bioinformatic methods, three genes TaTOB1, TaETT2, and TaDL are identified in bread wheat genome, their location is defined on the chromosomes of 2nd, 3rd and 4th homoeologous groups respectively. The polymorphisms between homoeoalleles of the genes located on A, B, and D subgenomes are described. Identified polymorphisms include variation in the length of the exons and intrones in all three genes, variation in the number of exons and intrones for the TaETT2 gene homoeoalleles, inversion of TaDL-B homoeoallele relative to the TaDL-A and TaDL-D, and inversion of TaETT2-B and TaETT2-D relative to the TaETT2-A. With the PCR method using primers designed to the sequence of the TaTOB1, the homoeoalleles of this gene were identified in the genomes Au, Ab, B, G, D, SSh, M, U, T of diploid, tetraploid, and hexaploid wheat species. The marker potential of two pairs of primers for the TaTOB1 gene is shown to study the genome structure of the introgressive wheat lines in relation to this gene.

Key words: bread wheat, awns, awn development genes, Aegilops, wheat genome sequence analysis

Tsitologiya i Genetika 2019, vol. 53, no. 4, pp. 3-12

E-mail: a.navalihina gmail.com, antonyuk.m ukma.edu.ua, t.pasichnyk ukma.edu.ua, ternovska ukma.edu.ua

Navalikhina A., Antonyuk M., Pasichnyk T., Ternovska T. Identification of Oryza sativa awn development regulatory genes orthologs in Triticinae accessions, Tsitol Genet., 2019, vol. 53, no. 4, pp. 3-12.

In "Cytology and Genetics":
A. Navalikhina, M. Antonyuk, T. Pasichnyk, T. Ternovska Identification of Oryza sativaТs Awn Development Regulatory Gene Orthologs in Triticinae Accessions, Cytol Genet., 2019, vol. 53, no. 4, pp. 267Ц275
DOI: 10.3103/S0095452719040091


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