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Potential role of protein kinases SnRK1 in regulation of cell division of Arabidopsis thaliana

Krasnoperova E.E., Buy D.D., Goriunova I.I., Isayenkov S.V., Karpov P.A., Blume Ya.B., Yemets A.I.

 




SUMMARY. It is well known that the SNF1-related protein kinase-1 (SnRK1) subfamily is involved in the regulation of carbohydrate metabolism and energy balance. These enzymes are multifunctional and can participate in many other important cellular processes. In this work, the role of protein kinases SnRK1 (KIN10 and KIN11) in the regulation of the cell division of Arabidopsis tha-liana was studied. For this purpose, A. thaliana kin10 and kin11 knockout lines (http://arabidopsis.info/) were used. The cells of these mutant lines exhibited the low mitotic index. The expression level of the cell pro-liferation markers – CYCB1, 1 (cyclin B) and plant BRCA1 homolog (Breast Cancer Suppressor Protein) was reduced too. A significantly smaller mitotic index and expression level of CYCB1, 1 and BRCA1 genes were found in the mutants that were grown under energy starvation conditions. High level of expression of CYCB1, 1/BRCA1 and KIN10/KIN11 genes in A. thaliana cell suspension culture was also revealed in comparison to Arabidopsis seedlings. Obtained data may indicate the possible role of protein kinases KIN10/KIN11 in regulation of cell proliferative activity.

Key words: protein kinases, Arabidopsis thaliana, SnRK1, KIN10, KIN11, gene expression, mitotic markers, cell division

Tsitologiya i Genetika 2019, vol. 53, no. 3, pp. 3-11

E-mail: krasnopio524 gmail.com, denisbuy90 gmail.com, inna.horiunova.ukr gmail.com, stan.isayenkov gmail.com, karpov.p.a gmail.com, cellbio cellbio.freenet.viaduk.net, yemets.alla gmail.com

Krasnoperova E.E., Buy D.D., Goriunova I.I., Isayenkov S.V., Karpov P.A., Blume Ya.B., Yemets A.I. Potential role of protein kinases SnRK1 in regulation of cell division of Arabidopsis thaliana, Tsitol Genet., 2019, vol. 53, no. 3, pp. 3-11.

In "Cytology and Genetics":
O. E. Krasnoperova, D. D. Buy, I. I. Goriunova, S. V. Isayenkov, P. A. Karpov, Ya. B. Blume, A. I. Yemets The Potential Role of SnRK1 Protein Kinases in the Regulation of Cell Division in Arabidopsis thaliana, Cytol Genet., 2019, vol. 53, no. 3, pp. 185–191
DOI: 10.3103/S0095452719030022


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