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Reproductive function of cows with different genotype by locus TNFα and estimating the fertility of sperm cells by DNA fragmentation method

Bimenova Zh.Zh., Ussenbekov Ye.S., Terletskiy V.P., Makashev Ye.K., Tyshchenko V.I., Kassymbekova Sh.N.

 




SUMMARY. SNP polymorphism of the promoter region of the TNFα gene at position 824 A→G in the Holstein cows of Bayserke-Agro LLP is represented by the following genetic variants: AA – 22,4 %, AG – 63,8 %, GG – 13,8 %, the frequency of alleles A and G was 0,54 and 0,46, respectively. In population under this study, the excessive number of the heterozygous genotype AG reached the value of 21,49, and in other genotypes there was a deficit of homozygous variants of GG and AA, by 11,16 and 10,32 individuals respectively. The traits of reproductive function were high in cows with GG genotype: the interval between calving and productive insemination was 259 days, the insemination index was 2,63, the proportion of animals inseminated after more than 91 days was at minimum value of 47,36 % in individuals with homozygous genotype GG. As an additional criterion for assessing the fertility of bull sperm, the DNA fragmentation method is recom-mended, the maximum permissible level of sperm cells with DNA fragmentation in frozen sperm is between 13,00 and 17,15 %.

Key words: promoter region of the TNFα gene, PCR-RFLP, reproductive function of cows, DNA fragmentation, bull sperm fertility

Tsitologiya i Genetika 2019, vol. 53, no. 1, pp. 51-58

E-mail: zhan_225 mail.ru, usen03 mail.ru, valeriter mail.ru, e_makashev mail.ru, tinatvi mail.ru, kasymbekova­s mail.ru

Bimenova Zh.Zh., Ussenbekov Ye.S., Terletskiy V.P., Makashev Ye.K., Tyshchenko V.I., Kassymbekova Sh.N. Reproductive function of cows with different genotype by locus TNFα and estimating the fertility of sperm cells by DNA fragmentation method, Tsitol Genet., 2019, vol. 53, no. 1, pp. 51-58.

In "Cytology and Genetics":
Zh. Zh. Bimenova, Ye. S. Ussenbekov, V. P. Terletskiy, Ye. K. Makashev, V. I. Tyshchenko, Sh. N. Kassymbekova Reproductive Function of Cows with Different Genotypes for TNFα Locus and Estimation of Sperm Fertility by the DNA Fragmentation Method, Cytol Genet., 2019, vol. 53, no. 1, pp. 42–48
DOI: 10.3103/S0095452719010043


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