TSitologiya i Genetika 2019, vol. 53, no. 2, 43-55
Cytology and Genetics 2019, vol. 53, no. 2, 132–142, doi: https://www.doi.org/10.3103/S0095452719020026

Differently green-synthesized CdS quantum dots: comparison of toxicity, antiproliferative effects and adhesion activity on human cells

Garmanchuk L.V., Nehelia A.O., Inomistova M., Khranovska N.M., Tolstanova G.M., Blume Ya.B., Yemets A.I.

Quantum dots (QDs) are typical II–VI semiconductor materials, owing to their unique optical properties and tunable photoluminescence, have received much interest in the field of electronic and biomedical applications. However, the cytotoxicity of QDs has become a major concern for their safe usage in bioimaging, intracellular delivery or tracking molecules and organells. We have previously developed a novel alternative approach for the production of CdS QDs extracellularly by the biological synthesis using bacteria Escherichia coli, fungi Pleurotus ostreatus and plant Linaria maroccana as biomatrices. Produced nanoparticles are stable and luminescent, have a range of sizes from 2 to 10 nm in diameter. In the present research we have evaluated cytotoxic/cytostatic effects, proliferative activity, adhesive potential of produced CdS nanoparticles using HeLa cells, tumor cells of human T-and B-lymphocytes, AGS cancer cell line by MTT-assay and flow cytometry methods. It has been found that QDs have reduced toxicity as compared to inorganic cadmium sulfide which makes them attractive as a new type of non-toxic luminescent probes for bioimaging applications in cytological studies.

Keywords: Quantum dots, cadmium sulfide, cytotoxicity, cancer cells, proliferative activity, adhesive potential

TSitologiya i Genetika
2019, vol. 53, no. 2, 43-55

Current Issue
Cytology and Genetics
2019, vol. 53, no. 2, 132–142,
doi: 10.3103/S0095452719020026

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References

1. Zhao, M.X. and Zeng, E.Z., Application of functional quantum dot nanoparticles as fluorescence probes in cell labeling and tumor diagnostic imaging, Nanoscale Res. Lett., 2015, vol. 10, pp. 1–9. https://doi.org/10.1186/s11671-015-0873-8

2. Rosentha, S.J., Chang, J.C., Kovtun, O., McBride, J.R., and Tomlinson, I.D., Biocompatible quantum dots for biological applications, Chem. Biol., 2011, vol. 18, no. 1, pp. 10–24. https://doi.org/10.1016/j.chembiol.2010.11.013

3. Medintz, I.L., Mattoussi, H., and Clapp, A.R., Potential clinical applications of quantum dots, Int. J. Nanomed., 2008, vol. 3, no. 2, pp. 151–167.

4. Hoshino, A., Fujioka, K., Oku, T., Nakamura, S., Suga, M., Yamaguchi, Y., Suzuki, K., Yasuhara, M., and Yamamoto, K., Quantum dots targeted to the assigned organelle in living cells, Microbiol. Immunol., 2004, vol. 48, no. 12, pp. 985–994.

5. Tamašauskaitė-Tamašiūnaitė, L., Grincienė, G., Šimkūnaitė-Stanynienė, B., Naruškevicius, L., Pakštas, V., Selskis, A., and Norkus, E., Preparation of CdS nanoparticles by microwave-assisted synthesis, Chemija, 2015, vol. 26, no. 3, pp. 193–197.

6. Wang, G.Z., Chen, W., Liang, C.H., Wang, Y.W., Meng, G.W., and Zhang, L.D., Preparation and characterization of CdS nanoparticles by ultrasonic irradiation, Inorg. Chem. Commun., 2001, vol. 4, no. 4, pp. 208–210.

7. Marchiol, L., Synthesis of metal nanoparticles in living plants, Ital. J. Agron., 2012, vol. 7, no. 3, pp. 274–282. https://doi.org/10.4081/ija.2012.e37

8. Borovaya, M.N., Naumenko, A.P., Matvieieva, N.A., Blume, Y.B., and Yemets, A.I., Biosynthesis of luminescent CdS quantum dots using plant hairy root culture, Nanocsale Res. Lett., 2014, vol. 9, pp. 1–7. https://doi.org/10.1186/1556-276X-9-686

9. Borovaya, M.N., Naumenko, A.P., Yemets, A.I., and Blume, Y.B., Stability of the CdS quantum dots, synthesized by the bacteria Escherichia coli, Rep. Natl. Acad. Sci. Ukraine, 2014, vol. 7, pp. 145–151.

10. Borovaya, M.N., Naumenko, A.P., Pirko, Y.V., Krupodorova, T.A., Yemets, A.I., and Blume, Y.B., Production of CdS quantum dots with the use of the fungus Pleurotus ostreatus, Rep. Natl. Acad. Sci. Ukraine, 2014, vol. 2, pp. 153–159.

11. Borovaya, M., Pirko, Y., Krupodorova, T., Naumenko, A., Blume, Y., and Yemets, A., Biosynthesis of cadmium sulphide quantum dots by using Pleurotus ostreatus (Jacq.) P. Kumm, Biotechnol. Biotechn. Equipm., 2015, vol. 29, no. 6, pp. 1156–1163. https://doi.org/10.1080/13102818.2015.1064264

12. Borovaya, M.N., Burlaka, O.M., Naumenko, A.P., Blume, Ya.B., and Yemets, A.I., Extracellular synthesis of luminescent CdS quantum dots using plant cell culture, Nanoscale Res. Lett., 2016, vol. 11, no. 1, pp. 1–8. https://doi.org/10.1186/s11671-016-1314-z

13. Mosmann, T., Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxic assays, J. Immun. Meth., vol. 65, nos. 1–2, pp. 55–63.

14. McDonald, J.H., Handbook of Biological Statistics, Baltimore, Maryland: Sparky House Publishing, 2008.

15. Kong, B., Seog, J.H., Graham, L.M., and Lee, S.B., Experimental considerations on the cytotoxicity of nanoparticles, Nanomedicine, 2011, vol. 6, pp. 929–941. https://doi.org/10.2217/nnm.11.77

16. Bendas, G. and Borsig, L., Cancer cell adhesion and metastasis: selectins, integrins, and the inhibitory potential of heparins, Int. J. Cell Biol., 2012, vol. 2012, p. 10. https://doi.org/10.1155/2012/676731

17. Goo, S., Choi, Y.J., Lee, Y., Lee, S., and Chung, H.W., Selective effects of curcumin on CdSe/ZnS quantum-dot-induced phototoxicity using UVA irradiation in normal human lymphocytes and leukemia cells, Toxicol. Res., 2013, vol. 29, no. 1, pp. 35–42. https://doi.org/10.5487/TR.2013.29.1.035

18. Ober-Blobaum, J.L., Engelhardt, G., Hebel, S., Rink, L., and Haase, H., Cadmium ions promote monocytic differentiation of humanleukemia HL-60 cells treated with 1a,25-dihydroxyvitamin D3, Biol. Chem., 2010, vol. 391, no. 11, pp. 1295–303. https://doi.org/10.1515/BC.2010.135

19. Zhou, J., Yang, Y., and Zhang, C., Toward biocompatible semiconductor quantum dots: from biosynthesis and bioconjugation to biomedical application, Chem. Rev., 2015, vol. 115, no. 21, pp. 11669–11717. https://doi.org/10.1021/acs.chemrev.5b00049

20. Katsumiti, A., Gilliland, D., Arostegui, I., and Cajaraville, M.P., Cytotoxicity and cellular mechanisms involved in the toxicity of CdS quantum dots in hemocytes and gill cells of the mussel Mytilus galloprovincialis, Aquat. Toxicol., 2014, vol. 153, pp. 39–52. https://doi.org/10.1016/j.aquatox.2014.02.003

21. Munari, M., Sturve, J., Frenzilli, G., Sandersd, M.B., Brunelli, A., Marcomini, A., Nigroc, M., and Lyons, B.P., Genotoxic effects of CdS quantum dots and Ag2S nanoparticles in fish cell lines (RTG-2), Mutat. Res. Gen. Toxicol. Environ. Mutagen., 2014, vols., 775–776, pp. 89–93. https://doi.org/10.1016/j.mrgentox.2014.09.003

22. Cho, S.J., Maysinger, D., Jain, M., Roder, B., Hackbarth, S., and Winnik, F.M., Long-term exposure to CdTe quantum dots causes functional impairments in live cells, Langmuir, 2007, vol. 23, no. 4, pp. 1974–1980. https://doi.org/10.1021/la060093j

23. Li, K.G., Chen, J.T., Bai, S.S., Wen, X., Song, S.Y., Yu, Q., Li, J., and Wang, Y.Q., Intracellular oxidative stress and cadmium ions release induce cytotoxicity of unmodifiedcadmium sulphide quantum dots, Toxicol. In Vitro, 2009, vol. 23, no. 6, pp. 1007–1013. https://doi.org/10.1016/j.tiv.2009.06.020

24. Hossain, Sk.T. and Mukherjee, S.K., Toxicity of cadmium sulfide (CdS) nanoparticles against Escherichia coli and HeLa cells, J. Hazard. Mater., 2013, vol. 260, pp. 1073–1082. https://doi.org/10.1016/j.jhazmat.2013.07.005

25. Galeone, A., Vecchio, G., Malvindi, M.A., Brunetti, V., Cingolani, R., and Pompa, P.P., In vivo assessment of CdSe-ZnS quantum dots: coating dependent bioaccumulation and genotoxicity, Nanoscale, 2012, vol. 4, no. 2, pp. 6401–6407. https://doi.org/10.1039/c2nr31826a

26. Protsenko, O.V., Dudka, O.A., Kozeretskaya, I.A., Inomystova, M.V., Borovaya, M.N., Pirko, Ya.V., Tolstanova, A.N., Ostapchenko, L.I., and Yemets, A.I., Estimation of toxicity and genotoxicity of CdS quantum dots synthesized with the help of biological matrices, Proc. Natl. Acad. Sci. Ukraine, 2016, vol. 4, pp. 111–117.

27. Borovaya, M.N., Burlaka, O.M., Yemets, A.I., and Blume, Ya.B., Biosynthesis of Quantum Dots and Their Potential Applications in Biology and Biomedicine, Springer International Publishing Switzerland, 2015, vol. 167, no. 24, pp. 339–362. https://doi.org/10.1007/978-3-319-18543-9_24