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Comparison of alleles at Gli-2 loci of common wheat by means of two-dimensional electrophoresis of gliadin

Metakovsky E.V., Melnik V.A., Redaelli R., Rodriguez-Quijano M.

 




SUMMARY. Electrophoretic mobility (EM) and molecular weight (MW) of some allelic variants of α- and β-gliadins contrlled by Gli-2 loci were compared by means of two-dimensional (APAGE × SDS) electrophoresis. Com-parison of α-gliadins of the alleles Gli-A2b and Gli-A2p, of β-gliadins of the Gli-B2b and Gli-B2c, and of β-gliadins of the Gli-D2b, Gli-D2c, Gli-D2j, and Gli-D2r indicated that a gliadin with lower EM had, has a rule, bigger MW which is known to depend on the length of the polyglutamine domain of gliadin of α-type. However, allelic variants of the α-gliadin encoded by Gli-D2b и Gli-D2e differ in EM but not in apparent MW. It might be caused by a substitution of some charged/uncharged aminoacids in the polypeptide of gliadin. Allele Gli-B2o which is very frequent in up-to-date common wheat germplasm originated probably by means of unequal crossingover. Some alleles at Gli-A2 is found to control completely different blocks of gliadins and therefore might come to common wheat from different genotypes of the polymorphic diploid donor of the A genome. The results indicate that the reason of the known more vast polymorphism of gliadins controlled by Gli-2 loci as compared with Gli-1 loci is the considerable difference of the structure, first, of Gli-1 and Gli-2 loci (Gli-2 loci have more expressed genes per locus) and, second, of genes encoding gliadins of α- and γ-types (α-gliadins are shown to contain a long polyglutamine sequences highly variable in their length).

Key words: common wheat, gliadin, alleles at Gli-2 loci, two-dimensional electrophorsis

Tsitologiya i Genetika 2018, vol. 52, no. 2, pp. 3-12

E-mail: emetakovsky gmail.com

Metakovsky E.V., Melnik V.A., Redaelli R., Rodriguez-Quijano M. Comparison of alleles at Gli-2 loci of common wheat by means of two-dimensional electrophoresis of gliadin, Tsitol Genet., 2018, vol. 52, no. 2, pp. 3-12.

In "Cytology and Genetics":
E. V. Metakovsky, V. A. Melnik, R. Redaelli, M. Rodriguez-Quijano Comparison of Alleles at Gli-2 Loci of Common Wheat by Means of Two-Dimensional Electrophoresis of Gliadin, Cytol Genet., 2018, vol. 52, no. 2, pp. 87–94
DOI: 10.3103/S0095452718020068


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