ISSN 0564-3783  



Main page
Contacts
Themes
Contents  
Themes
Subscription
Information to authors
Editorial board
Mobile version


In Russian

Export citations
UNIMARC
BibTeX
RIS





Effects of exogenous phytohormones on spore germination and morphogenesis of Polystichum aculeatum (L.) roth gametophyte in culture invitro

Babenko L.M., Romanenko K.O., Shcherbatiuk M.M., Vasheka O.V., Romanenko P.O.&Nbsp;, Negretsky V.A., Kosakivska I.V.

 




SUMMARY. The effects of exogenous phytohormones gibberellic acid (GA) and benzyl-aminopurine (BAP) on the spore germination and gametophyte morphogenesis of Polystichum aculeatum (L.) Roth in culture in vitro were studied. In the control, four stages of gametophyte morphogenesis were determined and their time periods were established. Spore germination and protonema formation of P. aculeatum occurred according to Vit-taria-type, prothalium development according to Aspidium-type. The spores germination percentage depended on the storage time duration. It was found that pre-storage spore germination was 8095 % in culture in vitro. Spores viability was within the range of 68 to 85 % after 46 month storage under lab conditions and did not exceed 20 % after 1.5 year of the storage period. High concentrations of exogenous GA3 (105
and 106 ) and BAP (105 ) were revealed to inhibit spore germination significantly whereas low concentrations (GA3 107108 ) had an insignificant stimulating effect or did not affect germination at all (BAP 106, 107, 108 ). All concentrations of exoge-nous BAP were demonstrated to inhibit the develop-ment of P. aculeatum gametophyte at the protonema stage that might due to the apical dominance removing. The power of an inhibiting effect directly depended on BAP concentrations. The formation of abnormal thalli of P. aculeatum gametophyte in the response to exogenous GA3 treatments was observed. That abnormal form caused by damage of cell growth by elongation. A direct interrelationship between GA3 concentrations
and level of morphological abnormalities and grade of thalli underdevelopment was demonstrated.

Key words: Polystichum aculeatum, gametophyte, protonema, prothallus, thallus, benzylaminopurine, BAP, gibberellic acid, GA3.

Tsitologiya i Genetika 2018, vol. 52, no. 2, pp. 39-50

E-mail: lilia.babenko gmail.com

Babenko L.M., Romanenko K.O., Shcherbatiuk M.M., Vasheka O.V., Romanenko P.O.&Nbsp;, Negretsky V.A., Kosakivska I.V. Effects of exogenous phytohormones on spore germination and morphogenesis of Polystichum aculeatum (L.) roth gametophyte in culture invitro, Tsitol Genet., 2018, vol. 52, no. 2, pp. 39-50.

In "Cytology and Genetics":
L. M. Babenko, K. O. Romanenko, M. M. Shcherbatiuk, O. V. Vasheka, P. O. Romanenko, V. A. Negretsky, I. V. Kosakivska Effects of Exogenous Phytohormones on Spore Germination and Morphogenesis of Polystichum aculeatum (L.) Roth Gametophyte in vitro Culture, Cytol Genet., 2018, vol. 52, no. 2, pp. 117126
DOI: 10.3103/S0095452718020032


Copyright© ICBGE 2002-2020 Coded & Designed by Volodymyr Duplij Modified 23.02.20