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Grapes pedigree verification by microsatellite analysis

Karastan O.M., Muliukina N.A., Papina O.S.

 




SUMMARY. Microsatellite DNA markers were used to identify and compare the genotypes of 80 samples of grape and their presumptive parental forms. Both presumptive parental forms were confirmed in the origin of 51 samples. Further statistical evaluation showed a greater probability of origin precisely from the parent-couple confirmed on the previous stage (ХхY), rather than from any random varieties that did not participate in the study. The index XxY varied from 103 to 1012. For four samples, one of the presumptive parents was not confirmed due to non-matching of the studied microsatellite loci allele profiles. Due to the physical absence of presumptive parental forms and the lack of information about the composition of their microsatellite profiles in the study of pedigree of 22 samples, the genotypes of the ancestral forms were used.

Key words: microsatellite loci, origin, pedigree, paternity analysis, grapes, V. vinifera L.

Tsitologiya i Genetika 2018, vol. 52, no. 5, pp. 13-27

E-mail: olga.karastan gmail.com; tairmna2005 ukr.net; papina_ ukr.net

Karastan O.M., Muliukina N.A., Papina O.S. Grapes pedigree verification by microsatellite analysis, Tsitol Genet., 2018, vol. 52, no. 5, pp. 13-27.

In "Cytology and Genetics":
O. M. Karastan, N. A. Muliukina, O. S. Papina Verification of Grape Pedigree by Microsatellite Analysis, Cytol Genet., 2018, vol. 52, no. 5, pp. 331–342
DOI: 10.3103/S0095452718050031


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