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Methyl jasmonate and nitric oxide in regulation of stomatal apparatus of Arabidopsis thaliana

Yastreb Т.О., Kolupaev Yu.Е., Kokorev А.I., Horielova Е.I., Dmitriev А.P.

 




SUMMASRY. Effect of methyl jasmonate, donors and antagonists of nitric oxide (NO) on the state of stomata of Arabidopsis thaliana plants (Col-0) was studied. Treatment of the epidermis of leaves with a 50–400 µM solution of methyl jasmonate (MJ) caused a decrease in size of stomatal aperture and percentage of open stomata. These effects were completely eliminated after preliminary treatment of epidermis cells with NO scavenger PTIO (2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide) and partially after treatment with inhibitors of animal NO synthase (NG-nitro-L-arginine methyl ester, L-NAME) and nitrate reductase (sodium tungstate). NO donors (L-arginine and sodium nitrite) in concentrations of 0,5–2,0 mM also caused a decrease in number of open stomata and size of stomatal aperture. Action of L-arginine on state of stomata was leveled by pretreatment of cells with L-NAME, and effects of sodium nitrite were eliminated by pre-action of sodium tungstate. A conclusion was made about a possible participation of nitrogen oxide, forming both along the pathway of L-arginine oxidation, and during nitrate/nitrite reduction, in regulation of stomatal apparatus, and about its role in realization of MJ effect on state of stomata in A. thaliana.

Key words: Arabidopsis thaliana, stomata, methyl jasmonate, nitric oxide, L- arginine, nitrite, signaling

Tsitologiya i Genetika 2018, vol. 52, no. 6, pp. 10-17

E-mail: plant_biology ukr.net, dmitriev.ap gmail.com

Yastreb Т.О., Kolupaev Yu.Е., Kokorev А.I., Horielova Е.I., Dmitriev А.P. Methyl jasmonate and nitric oxide in regulation of stomatal apparatus of Arabidopsis thaliana, Tsitol Genet., 2018, vol. 52, no. 6, pp. 10-17.

In "Cytology and Genetics":
T. O. Yastreb, Yu. E. Kolupaev, A. I. Kokorev, E. I. Horielova, A. P. Dmitriev Methyl Jasmonate and Nitric Oxide in Regulation of the Stomatal Apparatus of Arabidopsis thaliana, Cytol Genet., 2018, vol. 52, no. 6, pp. 400–405
DOI: 10.3103/S0095452718060129


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