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The influence of different concentrations of ascorbic, gibberellic acids and pH of medium on embryogenesis and regeneration in anther culture of spring triticale

Yerzhebayeva R.S., Abekova A.M., Ainebekova B.A., Urazaliyev K.R., Bazylova T.A., Daniyarova A.K., Bersimbayeva G.Kh.

 




SUMMARY. In this work studied the effect of ascorbic, gibberellic acids and pH of medium on embryogenesis and re-generation in anther culture of spring triticale. The re-search of androgenesis conducted on two model samples ЯТХ-327-11 and ЯТХ 18-11. According to the research revealed, that the addition of ascorbic acid antioxidant to induce embryogenesis at 2–8 mg/l concentration into the culture medium enhances the formation of androgen structures and regeneration of green plants. The addition of gibberellic acid into the culture medium to induce embryogenesis gave negative effects. The average yield of green plants in the anther culture of spring triticale was 2 pcs/100 anthers. According to the research received 277 green plants, spontaneous doubling of chromosomes recorded 26,7 %.

Tsitologiya i Genetika 2017, vol. 51, no. 6, pp. 41-49



Yerzhebayeva R.S., Abekova A.M., Ainebekova B.A., Urazaliyev K.R., Bazylova T.A., Daniyarova A.K., Bersimbayeva G.Kh. The influence of different concentrations of ascorbic, gibberellic acids and pH of medium on embryogenesis and regeneration in anther culture of spring triticale, Tsitol Genet., 2017, vol. 51, no. 6, pp. 41-49.




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