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Mutagenesis testing using the LacZ reporter activity of the reparation gene mus209 in Drosophilamelanogaster

Yasinskiy Y., Omelyanchuk L.V., Zhuk O.V., Kozeretska I.A.

 




We studied a set of Drosophila melanogaster strains that could be potentially suitable for testing a variety of mutagenic factors. Their genomes contained insertions of the enhancer trap P{lacW} in which the activity of the LacZ reporter is under the control of the reparation genes regulatory region. We demonstrated that the beta-galactosidase reporter, which is encoded by insertion of P{lacW} element in the gene mus209, is induced by irradiation in the cells of the salivary glands and wing imaginal discs. Despite the fact that the reporting coloration is not associated with the dose of radiation treatment, we found that the induction threshold of the reporter is different for these tissues. Thus, coloration in salivary glands is detectable after the dose of 200 rad and above, whereas the imaginal discs get colored with 500 rad and above. Thereby, multiple thresholds for induction of the reporter in the various tissues allow approximating the received dose.

Key words: mutagenic factors, D. melanogaster, LacZ reporter, radiation

Tsitologiya i Genetika 2016, vol. 50, no. 3, pp. 12-15

  • Institute of Biology National Taras Shevchenko University, Kyiv
  • Institute of Molecular and Cellular Biology, Siberian Branch of Russian Academy of Science, Novosibirsk

E-mail: yasins.yar gmail.com

Yasinskiy Y., Omelyanchuk L.V., Zhuk O.V., Kozeretska I.A. Mutagenesis testing using the LacZ reporter activity of the reparation gene mus209 in Drosophilamelanogaster, Tsitol Genet., 2016, vol. 50, no. 3, pp. 12-15.

In "Cytology and Genetics":
Y. Yasinskiy, L. V. Omelyanchuk, O. V. Zhuk, I. A. Kozeretska Mutagenesis testing using the LacZ reporter activity of the reparation gene mus209 in Drosophila melanogaster, Cytol Genet., 2016, vol. 50, no. 3, pp. 158161
DOI: 10.3103/S0095452716030105


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