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Multiplex PCR assay for detection of human somatotropin and interferon alpha2b genes in plant material

Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V.

 




Using transgenic plants as factories for production of physiologically active human proteins arouses special concern because occasional escape of such transgenes into environment may cause health problems. Creation of plant varieties producing pharmaceutically valuable proteins should be accompanied by development of detection methods suitable for controlling the transgene behavior. Here we describe a multiplex PCR protocol for revealing of two human genes (encoding growth hormone and interferon alpha2b) that have been successfully introduced into plant genomes. The primer pair designed for detection of human growth hormone coding sequence amplifies fragments of dif-ferent size from the full-length gene in the human genome and the intronless coding sequence usually used for plant transformation. Application of this primer pair may be recommended for ruling out false positive results due to sample contamination with human DNA. Such a control may be useful also in PCR analysis during establishing of transgenic plants carrying genes of human origin.

Key words: genetically modified plants, human growth hormone, interferon, multiplex PCR, somatotropin

Tsitologiya i Genetika 2015, vol. 49, no. 3, pp. 3-8

Institute of Cell Biology and Genetic Engineering NAS of Ukraine, Kyiv

E-mail: i-gerasimenko ukr.net

Gerasymenko I.M., Mazur M.G., Sheludko Y.V., Kuchuk N.V. Multiplex PCR assay for detection of human somatotropin and interferon alpha2b genes in plant material, Tsitol Genet., 2015, vol. 49, no. 3, pp. 3-8.




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