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Rating geno-and cytotoxic action of colloidal gold nanoparticles on the erythroid cells of the bone marrow and tumors in animals with ascitic Ehrlich carcinoma

Losovska Yu.Vanaleskina., L., Lukyanova N.Yu., Todor I.M., Chekhun V.F.

 




There have been studied the genotoxic and cytotoxic effects of gold nanoparticles (GNP) with diameter of 5 nm administered at doses of 30 and 60 μg/kg toward erythroid lineage cells from bone marrow (BM) and tumor cells from mice with Erhlich ascites carcinoma (EAC). Simultaneously in these animals treated with mentioned GNP doses, the changes of transmembrane potential of tumor cells have be-en analyzed. It has been shown that significant al-terations of cell morphology of erythroid lineage BM cells from animals with EAC could be observed upon the action of 30 μg/kg GNP in 48 hours after GNP administration, and the most significant ones – at GNP dose of 60 μg/kg independently on duration of exposure. Also it has been revealed that GNP at a dose of 60 μg/kg causes significant increase of micronuclei (MN) in tumor cells at the background of simultaneous decrease of transmembrane mito-chondrial potential and these effects enhance along with increase of treatment duration period. These re-sults allow propose a combined approach for evaluation of biotoxicity of materials during their trials in vitro and in vivo for generation of vector nanosystems.

Key words: Erhlich ascites carcinoma, tumor cells, erythroid lineage cells from bone marrow, gold nanoparticles, micronuclei, transmembrane mitochondrial potential

Tsitologiya i Genetika 2015, vol. 49, no. 1, pp. 52-60

E-mail: Lozovskaya.2012 mail.ru

Losovska Yu.Vanaleskina., L., Lukyanova N.Yu., Todor I.M., Chekhun V.F. Rating geno-and cytotoxic action of colloidal gold nanoparticles on the erythroid cells of the bone marrow and tumors in animals with ascitic Ehrlich carcinoma, Tsitol Genet., 2015, vol. 49, no. 1, pp. 52-60.




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