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Plants regeneration from hairy roots cultures of periwinkle Vinca minor L. and foxglove purple Digitalis purpurea L.

Lioshina L., Bulko O.

 




SUMMARY. Plants regenerated from hairy roots and calluses of foxglove purple and periwinkle have been obtained. It was found that organogenesis in hairy root culture occurs spontaneously on hormone-free medium but with different efficiencies. The frequency of direct shoot formation from root cultures was up to 60 % in Digitalis and 3,7 % in Vinca. Addition of 1 mg/l BА, 0,1 mg/l NAA and 5 % sucrose to B5 medium increased regenerative capacity of Vinca roots up to 19,1 %. Regenerated plants showed morphological features typically seen in Ri-transgenic plants. They include growth and plagiotropism of the root system, increased shoot formation, changed leaf morphology and short internodes.

Key words: hairy root, plant regeneration, Vinca minor L., Digitalis purpurea L.

Tsitologiya i Genetika 2014, vol. 48, no. 5, pp. 36-42

E-mail: llioshina ukr.net

Lioshina L., Bulko O. Plants regeneration from hairy roots cultures of periwinkle Vinca minor L. and foxglove purple Digitalis purpurea L., Tsitol Genet., 2014, vol. 48, no. 5, pp. 36-42.




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