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Genetic transformation of flax (Linum usitatissimum L.) with chimeric GFP-TUA6 gene for visualisation microtubule

Shysha E.N., Korhovyu V.I., Bayer G.Ya., Guzenko E.V., Lemesh V.A., Kartel N.A., Yemets A.I., Blume Ya.B.

 




The data of Agrobacterium-mediated transformation of some Linum usitatissimum cultivars zoned on the territories of Belarus and Ukraine with the plasmid carrying chimeric GFP-TUA6 gene and nptII gene as selectable marker conferring resistance to kanamycin are presented in this study. Transformation was affected by a number of factors including optical density (OD600), time of inoculation of explants with Agrobacterium and co-culture conditions. Transgenic nature of obtained lines was confirmed by PCR analysis. Expression of GFP-TUA6 gene was detected with confocal laser scanning microscopy. The obtained transgenic lines can be used for further functional studies the role of microtubules in the processes of building the flax fibres and resistance to wind.

Tsitologiya i Genetika 2013, vol. 47, no. 2, pp. 3-11

E-mail: elenashysha mail.ru

Shysha E.N., Korhovyu V.I., Bayer G.Ya., Guzenko E.V., Lemesh V.A., Kartel N.A., Yemets A.I., Blume Ya.B. Genetic transformation of flax (Linum usitatissimum L.) with chimeric GFP-TUA6 gene for visualisation microtubule, Tsitol Genet., 2013, vol. 47, no. 2, pp. 3-11.

In "Cytology and Genetics":
E. N. Shysha, V. I. Korhovyu, G. Ya. Bayer, E. V. Guzenko, V. A. Lemesh, N. A. Kartel, A. I. Yemets, Ya. B. Blume Genetic transformation of flax (Linum usaitatissimum L.) with the chimeric GFP-TUA6 gene for the visualization of microtubules, Cytol Genet., 2013, vol. 47, no. 2, pp. 63-69
DOI: 10.3103/S0095452713020096


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