TSitologiya i Genetika 2013, vol. 47, no. 3, 14-20
Cytology and Genetics 2013, vol. 47, no. 3, 138-144, doi: https://www.doi.org/10.3103/S0095452713030031

Establishment of in vitro culture, plant regeneration and genetic transformation of Camelina sativa

Yemets A.I., Boychuk Yu.N., Shysha E.N., Rakhmetov D.B., Blume Ya.B.

SUMMARY. The results on in vitro culture establishment, plantlet regeneration and rooting of Camelina sativa cultivar sample Peremozhets and cultivar Mirazh are presented. Effective concentrations of sterilizing agents and duration of plant material treatment were estimated. Phytohormone ratio, sucrose concentration in nutrient medium that induce effective formation of C. sativa shoots and NAA concentration for plantlet rooting have been established. The method of Agrobacterium-mediated transformation of Camelina by using binary vector pGH217 carrying reporter β-glucoronidase (gus) gene driven under 35S CaMV promoter and nos-terminator, and selective marker hpt gene conferring hygromycin-resistance in transgenic plant was elaborated.

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TSitologiya i Genetika
2013, vol. 47, no. 3, 14-20

Current Issue
Cytology and Genetics
2013, vol. 47, no. 3, 138-144,
doi: 10.3103/S0095452713030031

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