ISSN 0564-3783  



Main page
Contacts
Preview papers  
Themes
Contents  
Themes
Subscription
Information to authors
Editorial board
Mobile version


In Russian

Export citations
UNIMARC
BibTeX
RIS





Isolation and amplification сDNA of 8H07 gene conservative region of nematode heterodera schachtii with high relationship to its rape homolog

Tsygankova V.А., Andrusevich Ya.V., Ponomarenko S.P., Galkin A.P., Blume Ya.B.

 




SUMMARY. Original method of small regulatory si/miRNA isolation from plant cells was elaborated. PCR amplification of fragment cDNA 8H07 nematode Heterodera schachtii gene was carried out. Using Northern-blot method hybridization of plant si/miRNA with cDNA fragment of conservative region 8H07 gene the presence of their high homology is found out. The amplified cDNA fragment of nematode 8H07 gene in future will be used for creation recombinant gene with complementary antisense dsRNA sequence for increasing resistance of rape plants to parasitic nematodes.

Tsitologiya i Genetika 2012, vol. 46, no. 6, pp. 3-11

E-mail: vTsygankova ukr.net, cellbio cellbio.fre

Tsygankova V.А., Andrusevich Ya.V., Ponomarenko S.P., Galkin A.P., Blume Ya.B. Isolation and amplification сDNA of 8H07 gene conservative region of nematode heterodera schachtii with high relationship to its rape homolog, Tsitol Genet., 2012, vol. 46, no. 6, pp. 3-11.

In "Cytology and Genetics":
V. A. Tsygankova, Ya. V. Andrusevich, S. P. Ponomarenko, A. P. Galkin, Ya. B. Blume Isolation and amplification of cDNA from the conserved region of the nematode Heterodera schachtii 8H07 gene with a close similarity to its homolog in rape plants, Cytol Genet., 2012, vol. 46, no. 6, pp. 335-341
DOI: 10.3103/S0095452712060114


Copyright© ICBGE 2002-2018 Coded & Designed by Volodymyr Duplij Modified 22.04.18