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Features of lettuce transgenic plants with ifnα2b gene regenerated after Agrobacterium rhizogenesmediated transformation

Matvieieva N.A., Shahovsky .., Kuchuk .V.

 




SUMMARY. Hairy roots of lettuce Lactuca sativa and regenerated plants with interferon-α2b gene had been obtained via Agrobacterium rhizogenes-mediated transformation. According to the results of PCR and rt-PCR analyses the studied plants had ifn-α2b gene. The regenerated plants differed from the plants of wild type by elongated internodes, early flower-bearing stem formation and purple coloration of leaves in artificial illumination conditions.

Key words: Lactuca sativa, Agrobacterium rhizogenes, transformation, ifn-α2b gene

Tsitologiya i Genetika 2012, vol. 46, no. 3, pp. 27-32

E-mail: joyna56 gmail.com

Matvieieva N.A., Shahovsky .., Kuchuk .V. Features of lettuce transgenic plants with ifnα2b gene regenerated after Agrobacterium rhizogenesmediated transformation, Tsitol Genet., 2012, vol. 46, no. 3, pp. 27-32.

In "Cytology and Genetics":
N. A. Matvieieva, A. M. Shakhovskij, M. V. Kuchuk Features of lettuce transgenic plants with the ifn-α2b gene regenerated after Agrobacterium rhizogenes-mediated transformation, Cytol Genet., 2012, vol. 46, no. 3, pp. 150-154
DOI: 10.3103/S0095452712030073


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