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Hete- rogeneity of cell population of lymphoma NK/Ly and leukemia L-1210 according to carbohydrate structure of cell surface : immunocytochemical analysis of lectin binding

Lutsyk M.M., Yashchenko A.M., Kovalyshyn V.I., Prydatko O.E., Stoika R.S., Lootsik M.D.

 


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The heterogeneity of tumor cell populations according to binding of lectins from lentil (LcL), wheat germs (WGA), peanut (PNA) and concanavalin A was investigated on a model of murine Nemeth-Kellner lymphoma (NK/Ly) and leukemia L-1210. Bound lectins were detected by indirect immunochemical method using home obtained polyclonal antilectin antibodies and immunogold silver staining (IGSS) technique. Significant differences in binding of Con A were revealed between NK/Ly (67 % Con A+) and L-1210 (7,2 % Con A+) cells, while the differences in binding of other lectins with both types of tumor cells were not significant. A relatively high percentage of PNA+ cells was registered that can indicate a high degree of desialization of membrane glycoproteins.

Key words: lymphoma NK/Ly, leukemia L 1210, carbohydrates of cell surface, lectins

Tsitologiya i Genetika 2011, vol. 45, no. 2, pp. 3-9

E-mail: lootsik cellbiol.lviv.ua, stoika cellbio

Lutsyk M.M., Yashchenko A.M., Kovalyshyn V.I., Prydatko O.E., Stoika R.S., Lootsik M.D. Hete- rogeneity of cell population of lymphoma NK/Ly and leukemia L-1210 according to carbohydrate structure of cell surface : immunocytochemical analysis of lectin binding, Tsitol Genet., 2011, vol. 45, no. 2, pp. 3-9.

In "Cytology and Genetics":
M. M. Lutsik, A. M. Yashchenko, V. I. Kovalishin, O. E. Pridatko, R. S. Stoika, M. D. Lutsik Heterogeneity of the population of lymphoma NK/Ly and leukemia L-1210 cells according to the carbohydrate structure of cell surfaces: Immunocytochemical analysis of lectin binding, Cytol Genet., 2011, vol. 45, no. 2, pp. 65-69
DOI: 10.3103/S0095452711020071


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