TSitologiya i Genetika 2011, vol. 45, no. 2, 3-9
Cytology and Genetics 2011, vol. 45, no. 2, 65-69, doi: https://www.doi.org/10.3103/S0095452711020071

Hete- rogeneity of cell population of lymphoma NK/Ly and leukemia L-1210 according to carbohydrate structure of cell surface : immunocytochemical analysis of lectin binding

Lutsyk M.M., Yashchenko A.M., Kovalyshyn V.I., Prydatko O.E., Stoika R.S., Lootsik M.D.

The heterogeneity of tumor cell populations according to binding of lectins from lentil (LcL), wheat germs (WGA), peanut (PNA) and concanavalin A was investigated on a model of murine Nemeth-Kellner lymphoma (NK/Ly) and leukemia L-1210. Bound lectins were detected by indirect immunochemical method using home obtained polyclonal antilectin antibodies and immunogold silver staining (IGSS) technique. Significant differences in binding of Con A were revealed between NK/Ly (67 % Con A+) and L-1210 (7,2 % Con A+) cells, while the differences in binding of other lectins with both types of tumor cells were not significant. A relatively high percentage of PNA+ cells was registered that can indicate a high degree of desialization of membrane glycoproteins.

Keywords: lymphoma NK/Ly, leukemia L 1210, carbohydrates of cell surface, lectins

TSitologiya i Genetika
2011, vol. 45, no. 2, 3-9

Current Issue
Cytology and Genetics
2011, vol. 45, no. 2, 65-69,
doi: 10.3103/S0095452711020071

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