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Polyclonal antibodies against human cell-surface antigen CD34

Nikolaiev Iu.S., Gilchuk P.V., Gorbatiuk O.B., Flyak A.I., Labyntsev A.J., Irodov D.M., Kolibo D.V., Kordium V.A.

 


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An efficient and inexpensive laboratory approach for the generation and the purification of polyclonal antibodies to human antigen CD34 was developed. It was shown that cloned refolded and purified from Escherichia coli recombinant extracellular fragment of CD34 antigen retained immunogenic determinants of cell-surface expressed CD34. Immunization of mice with unglycosylated truncated recombinant protein elicit polyclonal antibodies specific for the native human antigen CD34. The antibodies generated are applicable for phenotyping of CD34+ cells using immunocytochemistry and flow cytometry assays.

Tsitologiya i Genetika 2011, vol. 45, no. 3, pp. 3-14



Nikolaiev Iu.S., Gilchuk P.V., Gorbatiuk O.B., Flyak A.I., Labyntsev A.J., Irodov D.M., Kolibo D.V., Kordium V.A. Polyclonal antibodies against human cell-surface antigen CD34, Tsitol Genet., 2011, vol. 45, no. 3, pp. 3-14.

In "Cytology and Genetics":
Yu. S. Nikolaiev, P. V. Gilchuk, O. B. Gorbatiuk, A. I. Flyak, A. J. Labyntsev, D. M. Irodov, D. V. Kolibo, V. A. Kordium Polyclonal antibodies against the human cell surface CD34 marker, Cytol Genet., 2011, vol. 45, no. 3, pp. 133-142
DOI: 10.3103/S0095452711030066


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