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Obtaining of transgenic french bean plants (Phaseolus vulgaris L.) resistant to the herbicide pursuit by Agrobacterium-mediated transformation

Nifantova S.N., Komarnickiy I.K., Kuchuk N.V.

 


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The transgenic plants of French bean (Phaseolus vulgaris) resistant herbicide Pursuit and kanamycin have been obtained. The genetic transformation was carried out with Agrobacterium tumefaciens strain LBA4404 containing binary vector carrying mutant ahas/als and selective nptII genes. Integration of the transgenes into plant genome was confirmed by polymerase chain reaction.

Key words: French bean (Phaseolus vulgaris L.), genetic transformation, Agrobacterium tumefaciens, Pursuit herbicide, ahas/als gene

Tsitologiya i Genetika 2011, vol. 45, no. 2, pp. 41-45

E-mail: sveta iicb.kiev.ua

Nifantova S.N., Komarnickiy I.K., Kuchuk N.V. Obtaining of transgenic french bean plants (Phaseolus vulgaris L.) resistant to the herbicide pursuit by Agrobacterium-mediated transformation, Tsitol Genet., 2011, vol. 45, no. 2, pp. 41-45.

In "Cytology and Genetics":
S. N. Nifantova, I. K. Komarnickiy, N. V. Kuchuk Obtaining of transgenic french bean plants (Phaseolus vulgaris L.) resistant to the herbicide pursuit by agrobacterium-mediated transformation, Cytol Genet., 2011, vol. 45, no. 2, pp. 97-100
DOI: 10.3103/S0095452711020113


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