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Effective agrobacterium-mediated transformation of chicory (Cichorium intybus L.) with Mycobacterium tuberculosis antigene ESAT6

Matvieieva N.A., Vasylenko M.Y., Shahovsky A.M., Bannykova M.O., Kvasko O.Y., Kuchuk N.V.

 


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The conditions of high efficient chicory transformation with Mycobacterium tuberculosis antigene SAT6 have been determined. Transformation frequency was up to 86 % when the cotyledons were cultivated within 3 days without cefotaxime and then 1 day without kanamycine. DNA PCR-analysis has shown the presence both of selective nptII and target esxA genes in all analysed plants. At the same time RT-PCR has shown the presence of nptII transcripts for eight analysed lines and esxA transcripts for only five analysed lines.

Tsitologiya i Genetika 2011, vol. 45, no. 1, pp. 11-17

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Matvieieva N.A., Vasylenko M.Y., Shahovsky A.M., Bannykova M.O., Kvasko O.Y., Kuchuk N.V. Effective agrobacterium-mediated transformation of chicory (Cichorium intybus L.) with Mycobacterium tuberculosis antigene ESAT6, Tsitol Genet., 2011, vol. 45, no. 1, pp. 11-17.

In "Cytology and Genetics":
N. A. Matvieieva, M. Y. Vasylenko, A. M. Shahovsky, M. O. Bannykova, O. Y. Kvasko, N. V. Kuchuk Effective Agrobacterium-mediated transformation of chicory (Cichorium intybus L.) with Mycobacterium tuberculosis antigene ESAT6, Cytol Genet., 2011, vol. 45, no. 1, pp. 7-12
DOI: 10.3103/S0095452711010038


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