ISSN 0564-3783  



Main page
Contacts
Preview papers  
Themes
Contents  
Themes
Subscription
Information to authors
Editorial board
Mobile version


In Russian

Export citations
UNIMARC
BibTeX
RIS





Development of specific and degenerated primers to CesA genes encoding flax (Linum usitatissimum L.) cellulose syntase

Grushetskaya Z.E., Lemesh V.A., Khotyleva L.V.

 


[Free Full Text (pdf)]Article Free Full Text (pdf)  

Cellulose synthase catalytic subunit genes, CesA, have been discovered in several higher plant species, and it has been shown that the CesA gene family has multiple members. HVR2 fragment of these genes determine the class specificity of the CESA protein and its participation in the primary or secondary cell wall synthesis. The aim of this study was development of specific and degenerated primers to flax CesA gene fragments leading to obtaining the class specific HVR2 region of the gene. Two pairs of specific primers to the certain fragments of esA-1 and esA-6 genes and one pair of degenerated primers to HVR2 region of all flax esA genes were developed basing on comparison of six CesA EST sequences of flax and full cDNA sequences of Arabidopsis, poplar, maize and cotton plants, obtained from GenBank. After amplification of flax cDNA, the bands of expected size were detected (201 and 300 b.p. for the esA-1 and esA-6, and 600 b.p. for the HVR2 region of esA respectively). The developed markers can be used for cloning and sequencing of flax esA genes, identifying their number in flax genome, tissue and stage specificity.

Key words: cellulose syntase, CesA, degenerated primers, sequencing

Tsitologiya i Genetika 2010, vol. 44, no. 4, pp. 3-8

E-mail: z.grushetskaya igc.bas-net.by

Grushetskaya Z.E., Lemesh V.A., Khotyleva L.V. Development of specific and degenerated primers to CesA genes encoding flax (Linum usitatissimum L.) cellulose syntase, Tsitol Genet., 2010, vol. 44, no. 4, pp. 3-8.




Copyright© ICBGE 2002-2017 Coded & Designed by Volodymyr Duplij Modified 20.09.17