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A parentage study of closely related Ukrainian wine grape varieties using microsatellite markers

Goryslavets S., Risovanna V., Bacilieri R., Hausman J.-F., Heuertz M.

 


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Four bred grapevine varieties released for commercial cultivation in Ukraine, namely Antey Magarachskii, Rubinovyi Magaracha, Granatovyi Magaracha and Rubin Golodrigi, and their putative parental forms were genotyped using six microsatellite loci. Genotypes were compared with breeding records to verify genetic relationships among varieties. Results of the analysis confirmed four of six parent-offspring relationships. Results of the analysis allow to assume that genotype Seyve Villard 20347 is the direct parent of Antey Magarachskii instead of its grandparent. The first-studied accession believed to be that of Granatovyi Magaracha was identified as impurity. In order to verify the parentage of Granatovyi Magaracha, rest accessions of that variety and its putative parent Antey Magarachskii were additionally genotyped at 13 nuclear loci and at three chloroplast loci. The parent-offspring relationship was confirmed, as all Granatovyi Magaracha accessions had a common allele with the parent variety Antey Magarachskii at each locus and the same chlorotype A. Different Granatovyi Magaracha accessions could have been obtained via vegetative propagation of two seedlings which arose from one crossing.

Key words: grapevine, ssr marker, microsatellite loci, genetic relationships

Tsitologiya i Genetika 2010, vol. 44, no. 2, pp. 29-37

  • National Institute for Vine and Wine Magarach; Ampelography, Breeding and Genetics of Grapevine, Yalta, Ukraine
  • Institut national de la recherche agronomique, Centre INRA de Montpellier 2 Place Viala, 34000 Montpellier, France
  • Centre de Recherche Public-Gabriel Lippmann; Environment and Agro-Biotechnology; rue du Brill 41; L-4422 Belvaux, Luxembourg
  • Universit Libre de Bruxelles; Evolutionary and Ecology cp160/12; av. F.D. Roosevelt 50; B-1050 Brussels, Belgium
  • Centre of Forest Research CIFOR-INIA; Forest Systems and Resources; ctra. de la Corua km 7.5; E-28040 Madrid, Spain

E-mail: goricvet_2 rambler.ru

Goryslavets S., Risovanna V., Bacilieri R., Hausman J.-F., Heuertz M. A parentage study of closely related Ukrainian wine grape varieties using microsatellite markers, Tsitol Genet., 2010, vol. 44, no. 2, pp. 29-37.




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