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Statistical analysis of distribution of antibody level against Mycobacterium bovis antigens for bovine tuberculosis diagnostics

Redchuk T.A., Korotkevich N.V., Kaberniuk A.A., Oliinyk O.S., Labyntsev A.J., Romaniuk S.I., Kolibo D.V., Busol V.O., Komisarenko S.V.

 


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Antibody responses to purified protein derivate PPD of tuberculin and to antigens MPB63 and MPB83 of Mycobacterium bovis were determined in bovine herd (94 adult animals). Statistical approach based on approximation by multiple Gaussians with Levenberg-Marquardt algorithm for analysis of antibody level distribution against antigens examined was provided. Our results confirm that indirect ELISA with recombinant MPB83 and MPB63 as well as conventional PPD could be used for test-systems development for detection of cow tuberculosis infection at the herd level.

Key words: Mycobacterium bovis, tuberculosis, immunodiagnostics, MPB83, MPB63

Tsitologiya i Genetika 2010, vol. 44, no. 5, pp. 23-30

E-mail: rtakyiv gmail.com

Redchuk T.A., Korotkevich N.V., Kaberniuk A.A., Oliinyk O.S., Labyntsev A.J., Romaniuk S.I., Kolibo D.V., Busol V.O., Komisarenko S.V. Statistical analysis of distribution of antibody level against Mycobacterium bovis antigens for bovine tuberculosis diagnostics, Tsitol Genet., 2010, vol. 44, no. 5, pp. 23-30.




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