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Identification of allele variants of cattle milk productivity genes using pcr anti-primer method

Rashydov A.N., Spyridonov V.G., Konoval O.N., Melnychuk M.D.

 


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In this work we have demonstrated two independent real-time PCR methods for detection single nucleotide polymorphism (SNP) of genes csn and acyl-CoA: diacylglycerol acyltransferase 1 (dgat) in cattle. We have analyzed 296 samples of milk production cattle of Ukrainian breeding. The genotype frequencies were 0,58, 0,34, 0,08 for csn gene and for dgat gene 0,7, 0,26, 0,04. High efficiency of so called anti-primer method was shown. Duration of anti-primer PCR reaction was about 22,5 hours only and provided full investigation of unknown gene allele.

Key words: anti-primer PCR, SNP, CSN, DGAT1, detection of genes, cattle breed

Tsitologiya i Genetika 2010, vol. 44, no. 5, pp. 13-17

E-mail: arashydov yahoo.com

Rashydov A.N., Spyridonov V.G., Konoval O.N., Melnychuk M.D. Identification of allele variants of cattle milk productivity genes using pcr anti-primer method, Tsitol Genet., 2010, vol. 44, no. 5, pp. 13-17.




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