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Molecular and structural-biological analysis of Nicotiana plumbaginifolia mutants for identification of the site on their β-tubulins of interaction with dinitroanilines and phosphorotioamidates

Yemets A.I., Baird W.V., Nyporko A.Yu., Swire-Clark G.A., Blume Ya.B.

 




The identification of point mutation locations on β-tubulin molecules of amiprophosmethyl- and trifluralin-resistant Nicotiana plumbaginifolia lines have discribed in the work. It was shown that in the first case this mutation is connected with the substitution of serine residue on proline in position 248; in the second case – with the substitution of phenilalanine on serine in position 317 of β-tubulin amino acid sequence. Three-dimensional models of β-tubulin molecule from Chlamydomonas with well-known location of mutations conferring dinitroaniline- and phosphorotioamidate resistance (substitution of lysine residue to methionine on position 350), and β-tubulin from Nicotiana plumbaginifolia have been reconstructed. On the basis of analysis of site of interaction with dinitroanilines and phosphorotioamides on Chlamydomonas β-tubulin molecule it was concluded that the revealed mutations on Nicotiana plumbaginifolia β-tubulin affect amino acid residues participating in formation of this site.

Tsitologiya i Genetika 2009, vol. 43, no. 5, pp. 69-76

E-mail: alyemets univ.kiev.ua

Yemets A.I., Baird W.V., Nyporko A.Yu., Swire-Clark G.A., Blume Ya.B. Molecular and structural-biological analysis of Nicotiana plumbaginifolia mutants for identification of the site on their β-tubulins of interaction with dinitroanilines and phosphorotioamidates, Tsitol Genet., 2009, vol. 43, no. 5, pp. 69-76.




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