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Characterization of glucokinase polymorphisms associated with maturity-onset diabetes of the young (MODY2) in Jordanian population

FAWZI AL-SHEYAB, EMRAN KHAMAISEH, MARWAN ABU HALAWEH, RAIDA W. KHALIL

 




Maturity-Onset Diabetes of the Young (MODY) is a monogenic form of Diabetes Mellitus (DM) characterized by an autosomal dominant inheritance, onset usually before 25 years of age and a primary defect in glucose-stimulated insulin secretion. Glucokinase (GCK) acts as a glucose sensor in the pancreatic beta cell and regulates insulin secretion. The mutation in the gene encoding GCK results in enzyme inactivation cause MODY2. Functional studies of naturally occurring GCK mutations associated with hyperglycaemia provide further insight into the biochemical basis of glucose sensor regulation. In this study 100 diabetic Jordanian patients with MODY2 phenotype and 150 Normal control subjects were screened for the presence of GCK gene mutations including the missense mutations at position Thr228Ala in exon 7, Gly299Arg in exon 8 and nonsense mutation Ser383Ter in exon 9, utilizing polymerase chain reaction with restriction fragment length polymorphism (PCR-RFLP) analysis. The results shows no Thr228Ala, Gly299Arg and Ser383Ter mutations were detected in both groups, which was differ from the results obtained for Italian and Caucasian from the Oxford region in UK MODY2 patients. Our data indicated that the previously studied mutations in Italian and Caucasian patients in the GCK gene are not common in MODY Jordanian population, suggesting a racial difference can be found in the frequency of the GCK polymorphism.

Key words: Jordanian diabetic MODY2; Glucokinase;GCK. Abbreviations: MODY2, maturity onset of the young; GCK, Glucokinase; BMI, body mass index; FPS, fasting blood sugar

Tsitologiya i Genetika 2009, vol. 43, no. 5, pp. 58-63



FAWZI AL-SHEYAB, EMRAN KHAMAISEH, MARWAN ABU HALAWEH, RAIDA W. KHALIL Characterization of glucokinase polymorphisms associated with maturity-onset diabetes of the young (MODY2) in Jordanian population, Tsitol Genet., 2009, vol. 43, no. 5, pp. 58-63.




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