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RAPD-analysis of soybean cell line with cross-resistance to tungstem and vanadium oxyanions

Tishchenko E.N., Mykhalska S.I., Sergeeva L.E.

 


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The effect of vanadium oxyanions on genome of soybean (Glycine max L., Merr.) in tungsten-resistant cell line was studied. The line is resistant to V5+-oxyanions. RAPD-amplicons with identical length are differentially synthesized from DNAs of tungsten-resistant cell line as well as of the initial culture was shown by using the lethal dose of oxyanions W6+ and then V5+. The presence of instable loci in soybean genome under different stressors is discussed.

Key words: soybean (Glycine max L., Merr.), RAPD-PCR, tungsten/ vanadium-resistant, cell selection

Tsitologiya i Genetika 2009, vol. 43, no. 4, pp. 39-44

E-mail: plant ifrg.kiev.ua

Tishchenko E.N., Mykhalska S.I., Sergeeva L.E. RAPD-analysis of soybean cell line with cross-resistance to tungstem and vanadium oxyanions, Tsitol Genet., 2009, vol. 43, no. 4, pp. 39-44.




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