ISSN 0564-3783  



Main page
Contacts
Preview papers  
Themes
Contents  
Themes
Subscription
Information to authors
Editorial board
Mobile version


In Russian

Export citations
UNIMARC
BibTeX
RIS





Molecular polymorphism of cellular lines of wheat resistant to culture filtrate Gaeumannomyces graminis var. tritici, and plant-regenerants from them

Bavol A.V., Dubrovna O.V.

 




DNA polymorphism of wheat cellular lines resistant to culture filtrate of G. graminis var. tritici and regenerated plants has been investigated using ISSR-analysis. Specific changes in DNA sequence were revealed in resistant calluses. It was established, that all resistant cellular lines differed by the presence of specific amplicons of 2347 bp (primer 5’-TCTCTCTCTCTCTCTCG-3’) and 1745 bp (primer 5’-AGAGAGAGAGAGAGAGTC-3’), as well as the absence of the amplicon of 1108 bp (primer 5’-ACACACACACACACACC-3’) from the initial callus and the callus not exposed to the action of selective factor. These changes have been also revealed in R0 and R1 plants.

Key words: Triticum aestivum L., cellular selection, culture filtrate of Gaeumannomyces graminis var. tritici, plant regeneration, ISSR-analyse

Tsitologiya i Genetika 2009, vol. 43, no. 5, pp. 28-34

E-mail: bavol1 rambler.ru

Bavol A.V., Dubrovna O.V. Molecular polymorphism of cellular lines of wheat resistant to culture filtrate Gaeumannomyces graminis var. tritici, and plant-regenerants from them, Tsitol Genet., 2009, vol. 43, no. 5, pp. 28-34.




Copyright© ICBGE 2002-2017 Coded & Designed by Volodymyr Duplij Modified 23.11.17