Sakhno L.A., Gocheva E.A., Komarnitsky I.K., Kuchuk N.V.

Stable expression of promoterless bar gene in transgenic rape plants

Cytology and Genetics 2008, v 42, number 1, 21-28

Phosphinothricin resistant plants of two rapeseed (Brassica napus L. var. oleifera DC.) spring industrial cultivars were obtained using Agrobacterium tumefaciens leaf disk transformation. Vector constructions contained the promoterless coding sequence of phosphinothricin acetyltransferase (bar) gene located between two inverted lox-sites (elements of Cre/lox recombination system of P1 phage) and selective neomycinphosphotransferase II gene (nptII). Integration of the alien genes was confirmed by the PCR analyses. Stable and linked inheritance of foreign genes in Т₁ and Т₂ progeny was shown.