ISSN 0564-3783  



Main page
Contacts
Preview papers  
Themes
Contents  
Themes
Subscription
Information to authors
Editorial board
Mobile version


In Russian

Export citations
UNIMARC
BibTeX
RIS





Phosphinothricin-resistant somatic hybrids Brassica napus+Orychophragmus violaceus

Sakhno L.A., Komarnitsky I.K., Cherep N.N., Kuchuk N.V.

 


[Free Full Text (pdf)]Article Free Full Text (pdf)  

between Brassica napus L. cv. Kalinovsky and Orychophragmus violaceus (L.) O.E. Shulz. were obtained as a result of somatic hybridization experiments. The hybrids inherited PPT resistance from O. violaceus plants which were previously transformed by the vector containing Spm/dSpm Zea mays transposon system with bar gene located within the nonautonomous transposon. The obtained plants had intermediate morphology. Their hybrid nature has been confirmed by isozyme (esterase and amilase activity) and PCR (bar, gus, Spm/dSpm integration) analyses. The hybrids combined B. napus plastom and O. violaceus mithochondrion that was revealed by PCRRFLP. The hybrid plants might be included to rapeseed breeding programme after examination of their oil quality as well as to chloroplast transformation experiments that is still urgent for B. napus.

Tsitologiya i Genetika 2007, vol. 41, no. 1, pp. 3-8

E-mail: iicb iicb.kiev.ua

Sakhno L.A., Komarnitsky I.K., Cherep N.N., Kuchuk N.V. Phosphinothricin-resistant somatic hybrids Brassica napus+Orychophragmus violaceus, Tsitol Genet., 2007, vol. 41, no. 1, pp. 3-8.




Copyright© ICBGE 2002-2018 Coded & Designed by Volodymyr Duplij Modified 24.09.18