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Plant mutant tubulin genes as marker selective genes for genetic engineering

Yemets A.I., Blume Ya.B.

 


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>The approaches for new marker genes usage in selection of transformed plant cells, which are based on using mutant tubulin genes from natural plant biotypes and, in perspective, from induced plant mutants have been considered. The results of investigations of plant (biotypes, mutants) resistance to herbicides with antimicrotubular mode of action on molecular and cellular levels have been summarized. The reports dealing with study the transferring and the expression of mutant tubulin genes conferring resistance to amiprophosmethyl (phosphorothioamidate herbicide) and to trifluralin (dinitroaniline herbicide) from corresponding N. plumbaginifolia mutants into related and remote plant species by somatic hybridisation methods have been analyzed. The results of experiments on monocotyledonous and dicotyledonous plant transformation by mutant α-tubulin gene conferring resistance to dinitroanilines are described to test the possibility of its using as a marker gene with obtaining, at the same time, a dinitroaniline-resistant plants.

Tsitologiya i Genetika 2007, vol. 41, no. 3, pp. 29-43



Yemets A.I., Blume Ya.B. Plant mutant tubulin genes as marker selective genes for genetic engineering, Tsitol Genet., 2007, vol. 41, no. 3, pp. 29-43.




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