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The effect of DNA reamplification on RAPD-PCR results

Gusenko E.V., Lemesh V.A., Khotyleva L.V.

 


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The results of reamplification of flax DNA with random primers are represented. It was shown that eight of 17 decameric primers used in the experiments had an reamplification effect: the intensity of the bands increased; new bands and/or new polymorphic bands appeared in the gel sample. The results confirm the effectiveness and necessity of reamplification in case of uncertain or ambiguous interpretation of RAPD analysis making possible to select the highly-informative primers.

Key words: amplification, reamplification, RAPD-PCR, L. usitatissimum

Tsitologiya i Genetika 2006, vol. 40, no. 5, pp. 16-20

E-mail: guzenko tut.by

Gusenko E.V., Lemesh V.A., Khotyleva L.V. The effect of DNA reamplification on RAPD-PCR results, Tsitol Genet., 2006, vol. 40, no. 5, pp. 16-20.




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