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Production of transgenic sugarbeet plants (Beta vulgaris L.) using Agrobacterium rhizogenes

Kishchenko E.M., Komarnitskii I.K., Kuchuk N.V.

 


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Normal phenotype sugarbeet plants transformed with Agrobacterium rhizogenes were produced using direct regeneration from explants withouth hairy root phase. Kanamycin resistant plants and Ri-roots carrying the genes of neomycin phosphotransferase II and b-glucuronidase have been obtained. Integration of transgenes into sugarbeet genome was confirmed with GUS-assay and PCR using primers for the introduced genes.

Tsitologiya i Genetika 2005, vol. 39, no. 1, pp. 9-13



Kishchenko E.M., Komarnitskii I.K., Kuchuk N.V. Production of transgenic sugarbeet plants (Beta vulgaris L.) using Agrobacterium rhizogenes, Tsitol Genet., 2005, vol. 39, no. 1, pp. 9-13.




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