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Genetic variability among Solanum and Lycopersion species revealed by amplified fragment restriction polymorphysm analysis of chloroplast DNA

Komarnytskyy S.I., Komarnytskyy I.K.

 


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A phylogenetic cladogram for 23 species of genera Solanum and Lycopersion was constructed based on restriction analysis of 10 amplified regions of chloroplast DNA. Three major clusters were detected. All Lycopersicon species and S. lycopersicoides formed the first group. Second cluster was occupied by S. verrucosum, polyploid species of series Longipedicellata, and all dyploid and polyploid species native to South America. Two diploid series Pinnatisect and Bulbocastana were groupped together in the last claster. Previous morphological and molecular markers strongly support this type of phylogenetic analysis. Based on presented results it is evident that combination of chloroplast DNA region amplification and restriction could be a valuable alternative to a standard - RFLP analysis

Tsitologiya i Genetika 2004, vol. 38, no. 5, pp. 9-15



Komarnytskyy S.I., Komarnytskyy I.K. Genetic variability among Solanum and Lycopersion species revealed by amplified fragment restriction polymorphysm analysis of chloroplast DNA, Tsitol Genet., 2004, vol. 38, no. 5, pp. 9-15.




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