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Cytogenetic investigation of geranium plants obtained in tissue culture

Potemkina N.V., Egorova N.A., Bugara A.M.

 




Cytogenetic study of six cultivars and six selection lines of geranium (Pelargonium spp.), as well as of 100 plants regenerated from callus cultures has been performed. The majority of cultivars and lines had somatic chromosome numbers 2n = 7х = 56. Among regenerated plants of different cultivars (Rozovaja, Dushistaja, Krunk, Aist, Regar) obtained in vitro from various types of explants (internode, petiole) 61 % of diploids and 39 % aneuploids were revealed. Chromosome numbers in aneuploids varied from 46 to 82, among them 25,6 % regenerated plants had 2n = 72; 10,2 % - 2n = 68; 5,1 % - 2n = 64 and 12,8 % - 2n = 62. Addition of colhicine to nutrient medium increased the number of aneuploid plants.

Tsitologiya i Genetika 2004, vol. 38, no. 2, pp. 26-30



Potemkina N.V., Egorova N.A., Bugara A.M. Cytogenetic investigation of geranium plants obtained in tissue culture, Tsitol Genet., 2004, vol. 38, no. 2, pp. 26-30.




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